Regio Zuid-Holland | CryoGrid

Electron microscopy utilizes electrons instead of light to visualize molecules up to the atom-scale. Electron microscopy has gained significant traction within biology for visualizing the three-dimensional (3D) structure of proteins, increasing the understanding of its role and function within health and disease. Cryogenic electron microscopy (Cryo-EM), a technique that freezes or ""vitrifies"" a sample before imaging, is particularly suited to study the 3D structure of proteins. There is an unmet need for improved sample carriers to enhance the applicability of Cryo-EM. To allow high throughput studies with high reproducibility and reliability. DENSsolutions and CryoSol-World, aim to improve the current standard for Cryo-EM sample preparation by developing a novel sample carrier based on nanomanufacturing techniques and integrating the sample carrier within the VitroJet, a machine developed by CryoSol-World that automates sample deposition and vitrification of sample carriers..The novel sample carrier has enhanced geometry and material properties. It will be completely flat with known surface conditions, resulting in more reproducible layer thickness; especially in combination with pin-printing. By utilizing micro-electromechanical systems (MEMS) nanomanufacturing technologies, optimal control of production parameters can be achieved, leading to a significant reduction in variation between batches (e.g., surface inconsistencies, contamination, and graphene inconsistencies) and shorter lead times. The partnership combines unique expertise and knowledge surrounding MEMS-based sample carriers and Cryo-EM sample preparation. Accelerating the application of Cryo-EM within the .pharmaceutical industry and universities. The MIT R&D subsidy is crucial for the realization of this project and can be justified due to the intended innovation's contribution to the understanding of the function and role of proteins in both health and disease, and the development of novel drugs.